Explore further More information: Quantum dot/antibody conjugates for in vivo cytometric imaging in mice, Hee-Sun Han, PNAS, DOI: 10.1073/pnas.1421632111AbstractMultiplexed, phenotypic, intravital cytometric imaging requires novel fluorophore conjugates that have an appropriate size for long circulation and diffusion and show virtually no nonspecific binding to cells/serum while binding to cells of interest with high specificity. In addition, these conjugates must be stable and maintain a high quantum yield in the in vivo environments. Here, we show that this can be achieved using compact (∼15 nm in hydrodynamic diameter) and biocompatible quantum dot (QD) -Ab conjugates. We developed these conjugates by coupling whole mAbs to QDs coated with norbornene-displaying polyimidazole ligands using tetrazine–norbornene cycloaddition. Our QD immunoconstructs were used for in vivo single-cell labeling in bone marrow. The intravital imaging studies using a chronic calvarial bone window showed that our QD-Ab conjugates diffuse into the entire bone marrow and efficiently label single cells belonging to rare populations of hematopoietic stem and progenitor cells (Sca1+c-Kit+ cells). This in vivo cytometric technique may be useful in a wide range of structural and functional imaging to study the interactions between cells and between a cell and its environment in intact and diseased tissues. Citation: Quantum dots combined with antibodies as a method for studying cells in their native environment (2015, January 27) retrieved 18 August 2019 from https://phys.org/news/2015-01-quantum-dots-combined-antibodies-method.html Shining a light on quantum dots measurement
Cascading waterfalls, mighty mountains, wild forests and the lush green valleys make for everyone’s idea of utopia. For some it is a reality that they breathe day in and day out. The Himalayas, nurtures and sustains the faiths and socio-religious beliefs of its civilization. It cradles myths, legends and tales – some told some untold. Author Alka Raghuvanshi and photographer Sanjay Sharma capture these magical moments in places few have ventured due to reasons of inaccessibility in the book Also Read – ‘Playing Jojo was emotionally exhausting’ Garhwal Himalayas- Chorus of Solitude. ‘Perhaps it was ordained that many of the trips were made well before the book was even envisaged. And as the reader goes through the maze of legends and reality juxtaposed in weird and wonderful patterns, it would be important to remember that this journey into the unforgiving terrain – one wrong move is all that separates life from life eternal,’ says the author Alka Raghuvanshi. The photographer made endless trips to most places featured in this book in different seasons in his quest for getting just the right picture, including earlier this year in adverse weather conditions. Being the last documentation of the Uttrakhand region before havoc, this book written over six years assumes even more importance in this context.
Heavily armed fighters in all-terrain vehicles stormed the town of Kukawa, some 180 kilometres (112 miles) from the Borno state capital, Maiduguri, and opened fire on police and a local market.Kukawa, near Lake Chad, has been repeatedly targeted by Boko Haram, forcing Nigeria’s state-run oil company to abandon prospecting and drilling.The latest attack happened on Monday and was slow to emerge because telecommunications in Borno have been largely destroyed by five years of violence. Also Read – Pro-Govt supporters rally as Hong Kong’s divisions deepenThey (the gunmen) killed several people, especially around the market, where traders had gone for commercial activities,’ the Kukawa local government chairman Modu Musa ‘They burnt the whole market, the police station, government lodge, dozens of vehicles and most houses in the town in indiscriminate rocket and bomb attacks.’Police officers in Kukawa initially intercepted the insurgents on the outskirts of the town and engaged them in a fight but were forced to retreat because of the gunmen’s superior firepower. Also Read – Pak Army ‘fully prepared’ to face any challenge: Army spokesmanHundreds of residents fled to Maiduguri, joining tens of thousands of others who have abandoned their homes and livelihoods as a result of sustained attacks in Borno and two neighbouring states. Nigeria’s government earlier this month announced that they had secured a ceasefire deal with Boko Haram and an agreement to release 219 schoolgirls abducted from their Borno school in mid-April.But violence and further kidnappings have continued unabated.
17th Bharat Rang Mahotsav is about plays from across the world. But apart from these plays, 240 ambience performances by around 50 groups have been scheduled to thrill the audience non-stop at the NSD’s open courtyard. The ambience theatre in National school of Drama is an invitation to folk artistes, students from various institutions like DU, JNU, Jamia Milia Islamia and many more. It is related to gender issues, elections, women safety, harmony and other issues of social importance. These issues will be portrayed in various performances like Nukadnatak (Street Play), Katputli Dance (Puppet Dance) along with varied folk performances. Also Read – ‘Playing Jojo was emotionally exhausting’The ambience theatre is accessible to all at free of cost. Nearly 30 colleges, 15 different folk troupes and 8 new shows of Theatre In Education Company (TIE) will be a part of this new venture, with 4 to 5 shows per group. This endeavor was an initiative by the National School of Drama under the guidance of Amitesh Grover, Assistant Professor, National School of Drama and Naresh, President of NSD Student Union.“The basic idea to behind this concept was to generate interest and to connect the youth to the world of theatre that will help in creating an atmosphere for better theatre productions. Various colleges and educational institution are taking part in it,” said Amitesh Grover.When: On till February 18 Where: NSD’s open courtyard
Kolkata: As many as 13 people were killed and 20 others injured in lightning strikes in various parts of the state on Monday morning.A nor’wester lashed different districts, accompanied by lightning, resulting in death of the victims, who were mostly farmers. They were working in the fields when the accident occurred.Among the deceased, three persons were from Murshidabad, Nadia and North 24-Parganas each, while four others were from Dakshin Dinajpur and Malda, two each from each district. Also Read – Heavy rain hits traffic, flightsAccording to a senior official of the disaster management department, most of the victims were out on streets or fields, when they were struck by lightning. It has been learnt that among the injured, four persons from Purulia are stated to be in critical condition.In view of the warnings issued by the weather office, the district administrations have alerted all the blocks, asking them to take precautionary measures so that such incidents can be averted. The villagers in various blocks have been asked not to work out on the field during a thundershower. Also Read – Speeding Jaguar crashes into Merc, 2 B’deshi bystanders killedSeveral parts of South Bengal districts remained overcast from the early Monday morning. There was heavy downpour in the districts of Birbhum, Bankura, East and West Burdwan, Jhargram and Midnapore. The city and its adjoining areas in North and South Bengal also received rainfall on Monday morning. No damage or casualty has however been reported from the city.According to the weather office, a wind measuring around 60 km/h hit various districts, along with the rain bringing down the temperature by a few notches. People from the city have also breathed a sigh of relief, after respite from the sultry and uncomfortable weather. The commuters in the city and suburban areas also faced difficulties in reaching their destinations due to rain accompanied by storm. The regional meteorological centre at Alipore has predicted thundershower in the South Bengal districts in the next 48 hours.Heavy rain and storm have affected train services in various sections of Howrah and Sealdah divisions of Eastern Railway on Monday morning. Many of the local trains were delayed due to safety precautions.Train services on the Krishnanagar-Lalgola section were affected since 9.33 am, as some debris fell on the overhead wire at Bethuadahari. As a result of the incident, 13113 UP Kolkata-lalgola Hazarduari Express was detained near Bethuadahari. A tower van was pressed into action for the restoration of train services in the section.
In second such incident in the last three days, three unidentified men uprooted an unguarded ATM machine and looted cash around Rs 17.5 lakh in north Delhi’s Wazirabad, police said on Thursday.Yogendra Kumar, the person in charge of guarding the Corporation Bank ATM, first raised alarm at around 6 am by contacting a field officer of the bank who further informed the police.According to the police, the accused, who came riding a mini truck, struck the installation at around 2 am on Thursday. “The thieves executed the operation within a matter of a few minutes by covering the CCTV cameras inside the cabin with tapes. But some other CCTV cameras around the ATM machine enclosure captured the faces of the suspects,” a police officer said.Police added that the ATM, that remains unguarded from 11 pm to 6 am, was full of cash as it was replenished on Thursday.
Launched in 2008, the fair has established itself as South Asia’s leading platform for modern and contemporary art. The upcoming addition of the fair will take place from 28 to 31 January 2016. Building on these foundations, the fair is revising its mission and refining its programming to reflect the best of South Asia’s diversity in the visual arts. It is making a number of enhancements in the organisation which includes significant changes to its partnerships, internal team and gallery programming. Also Read – ‘Playing Jojo was emotionally exhausting’BMW is the new Presenting Partner of India Art Fair
If relationships are good, use of both positive and negative humour by leaders can help improve their subordinates’ job satisfaction, suggests new research.“Generally, people think that positive humour, which is inclusive, affiliative and tasteful, is good in leadership, and negative humour, which is aggressive and offensive, is bad,” said one of the researchers Christopher Robert, associate professor at University of Missouri in the US.“In our study, we found the effects of humour depend on the relationship between leaders and subordinates,” Robert noted. Also Read – ‘Playing Jojo was emotionally exhausting’Specifically, both positive and negative humour use by leaders is positively related to their subordinates’ job satisfaction when the relationship between the leader and subordinates is good. However, when the leader-subordinate relationship is bad, both negative and positive types of humour are associated with lower job satisfaction. In other words, for leaders, sometimes good humour has bad effects and bad humour has good effects on subordinates. Also Read – Leslie doing new comedy special with NetflixTo test their theory, the researchers developed two sets of matched questionnaires, one for leaders and one for their subordinates. They analysed responses from about 70 leaders and their 241 subordinates in 54 organisations. “The findings suggest that if leaders wish to integrate humour into their interactions with subordinates, they should first assess whether or not their subordinates are likely to interpret their